Basic Vector Information
- Vector Name:
- pHSV-TK
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3007 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Insect cells
- Promoter:
- SP6,T7
- 5' Primer:
- M13F
- Growth Strain(s):
- DH10B
- Growth Temperature:
- 37℃
pHSV-TK vector Map
pHSV-TK vector Sequence
LOCUS . 3007 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
primer_bind 35..51
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 67..85
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal complement(126..174)
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter complement(221..239)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(246..262)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
terminator 404..431
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
rep_origin 457..912
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 990..1094
/label="AmpR promoter"
CDS 1095..1952
/label="AmpR"
/note="β-lactamase"
rep_origin 2126..2714
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(2900..2971)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
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