pMEL10 vector (V017723)

LOCUS       .                       6103 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      229..1211
                     /label="2μ ori"
                     /note="yeast 2μ plasmid origin of replication"
     promoter        1238..1342
                     /label="AmpR promoter"
     CDS             1343..2200
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2374..2962
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3250..3271
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3286..3316
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3324..3340
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     3348..3364
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3385..3403
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3421..3689
                     /label="SNR52 promoter"
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
     misc_RNA        3710..3784
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      3789..3808
                     /label="SUP4 terminator"
                     /note="transcription terminator for the S. cerevisiae SUP4
                     tRNA gene"
     terminator      3814..4061
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(4080..4098)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4108..4124)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4597..4630)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."