Cart 2

pWB980-PgtE-6×His-p15A vector (V017720)

Basic Vector Information

Vector Name:
pWB980-PgtE-6×His-p15A
Antibiotic Resistance:
Ampicillin
Length:
6284 bp
Type:
Protein expression, Secretory expression
Replication origin:
p15A ori
Host:
Bacillus subtilis
Copy Number:
Low
Promoter:
P43
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pWB980-PgtE-6×His-p15A vector Map

Copy Sequence View Map
pWB980-PgtE-6×His-p15A6284 bp30060090012001500180021002400270030003300360039004200450048005100540057006000repBAmpR promoterAmpRp15A ori6xHis

pWB980-PgtE-6×His-p15A vector Sequence

LOCUS       .                       6284 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             309..1310
                     /label="repB"
                     /note="RepB replication protein"
     promoter        2309..2400
                     /label="AmpR promoter"
     CDS             2401..3258
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3336..3881
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             6208..6225
                     /label="6xHis"
                     /note="6xHis affinity tag"

This page is informational only.