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pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X4-6×His vector (V017718)

Basic Vector Information

Vector Name:
pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X4-6×His
Antibiotic Resistance:
Ampicillin
Length:
10931 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
Selection Marker:
HIS4,Neo/G418
Promoter:
AOX1
5' Primer:
3'AOX
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X4-6×His vector Map

Copy Sequence View Map
pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X4-6×His10931 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500AOX1 promoterα-factor secretion signal6xHisAOX1 terminatorKanRAOX1 3' fragmentbomoriAmpRAmpR promoter

pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X4-6×His vector Sequence

LOCUS       .                      10931 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        2..937
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             949..1215
                     /label="α-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     CDS             2935..2952
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2976..3222
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     CDS             complement(6586..7398)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7777..8533
                     /label="AOX1 3' fragment"
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    8676..8816
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9002..9590)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9764..10621)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(10622..10726)
                     /label="AmpR promoter"

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