pMEL16 vector (V017716)

LOCUS       .                       5772 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      155..1137
                     /label="2μ ori"
                     /note="yeast 2μ plasmid origin of replication"
     promoter        1164..1268
                     /label="AmpR promoter"
     CDS             1269..2126
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2300..2888
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3176..3197
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3212..3242
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3250..3266
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     3274..3290
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3347..3615
                     /label="SNR52 promoter"
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
     misc_RNA        3636..3710
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      3715..3734
                     /label="SUP4 terminator"
                     /note="transcription terminator for the S. cerevisiae SUP4
                     tRNA gene"
     terminator      3740..3987
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(4006..4024)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4034..4050)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4728..5387)
                     /label="HIS3"
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     promoter        complement(5388..5574)
                     /label="HIS3 promoter"