Basic Vector Information
- Vector Name:
- pU6-MCS-2×sgRNA-Cas9-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9650 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Puro
- Promoter:
- U6
- 5' Primer:
- BGH-R
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pU6-MCS-2×sgRNA-Cas9-Puro vector Map
pU6-MCS-2×sgRNA-Cas9-Puro vector Sequence
LOCUS . 9650 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_RNA 337..412
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 503..743
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
enhancer 943..1228
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1230..1507
/label="chicken β-actin promoter"
CDS 1873..5973
/label="Cas9"
/note="S. pyogenes Cas9, codon optimized for C. elegans
with synthetic introns"
polyA_signal 6715..6922
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
repeat_region 6931..7071
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 7146..7601
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7883..7987
/label="AmpR promoter"
CDS 7988..8845
/label="AmpR"
/note="β-lactamase"
rep_origin 9019..9607
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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