Basic Vector Information
- Vector Name:
- pTac-3×FLAG-β-Gal-SecM
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7492 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- pTac
- 5' Primer:
- AmpNR
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pTac-3×FLAG-β-Gal-SecM vector Map
pTac-3×FLAG-β-Gal-SecM vector Sequence
LOCUS . 7492 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 183..211
/label="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
CDS 261..326
/label="3xFLAG"
/note="three tandem FLAG® epitope tags, followed by an
enterokinase cleavage site"
CDS 339..3410
/label="lacZ"
/note="β-galactosidase"
promoter 3795..3899
/label="AmpR promoter"
CDS 3900..4757
/label="AmpR"
/note="β-lactamase"
rep_origin 4931..5519
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 5763..5840
/label="lacIq promoter"
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 5841..6920
/label="lacI"
/note="lac repressor"
protein_bind 6936..6957
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6972..7002
/label="lac promoter"
/note="promoter for the E. coli lac operon"
CDS 7046..7219
/label="lacZα"
/note="LacZα fragment of β-galactosidase"
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