Basic Vector Information
- Vector Name:
- pGAP-MCS-TEF1-MCS-URA3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6059 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Yeast
- Selection Marker:
- URA3
- Promoter:
- GAP,TEF1
- 5' Primer:
- AmpNR
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pGAP-MCS-TEF1-MCS-URA3 vector Map
pGAP-MCS-TEF1-MCS-URA3 vector Sequence
LOCUS . 6059 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
terminator complement(13..178)
/label="ADH1 terminator"
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
promoter complement(369..768)
/label="TEF1 promoter"
/note="promoter for EF-1α"
promoter complement(785..803)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 823..1466
/label="GAP promoter"
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
primer_bind 1472..1488
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1522..1538)
/label="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
terminator 1541..1788
/label="CYC1 terminator"
/note="transcription terminator for CYC1"
promoter 1958..2178
/label="URA3 promoter"
CDS 2179..2979
/label="URA3"
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
CDS 3616..3804
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 3909..4049
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(4235..4823)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4997..5854)
/label="AmpR"
/note="β-lactamase"
promoter complement(5855..5959)
/label="AmpR promoter"
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