Basic Vector Information
- Vector Name:
- pUC57-Tac-ECFP-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4463 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- T7
- 5' Primer:
- PUC57-F1（2023.10.26）
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pUC57-Tac-ECFP-EGFP vector Map
pUC57-Tac-ECFP-EGFP vector Sequence
LOCUS . 4463 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
primer_bind 379..395
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(435..512)
/label="lacI promoter"
promoter 558..586
/label="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
CDS 600..1316
/label="ECFP"
/note="enhanced CFP"
terminator 2100..2147
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(2242..2258)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2266..2282)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(2290..2320)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(2335..2356)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2644..3232)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3406..4263)
/label="AmpR"
/note="β-lactamase"
promoter complement(4264..4368)
/label="AmpR promoter"
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