Basic Vector Information
- Vector Name:
- pKD46-Tac-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7166 bp
- Type:
- Gene-editing vector
- Replication origin:
- pSC101 ori
- Host:
- E. coli
- Promoter:
- araBAD
- 5' Primer:
- G35612-F1：CTTTAGTCAGTTCCGCAGTATT
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
pKD46-Tac-EGFP vector Map
pKD46-Tac-EGFP vector Sequence
LOCUS . 7166 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS complement(14..889)
/label="araC"
/note="L-arabinose regulatory protein"
promoter 916..1200
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 1244..1657
/label="Gam"
/note="inhibitor of the host RecBCD nuclease in the λ Red
system"
CDS 1666..2448
/label="Beta"
/note="single-stranded DNA binding recombinase in the λ Red
system"
CDS 2448..3125
/label="Exo"
/note="5' to 3' double-stranded DNA exonuclease in the λ
Red system"
terminator 3129..3373
/label="λ tL3 terminator"
/note="transcription terminator tL3 from phage λ"
CDS complement(3499..4446)
/label="Rep101"
/note="RepA protein needed for replication with the pSC101
origin"
rep_origin complement(4494..4716)
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
promoter 4884..4912
/label="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
CDS 4959..5675
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
CDS complement(6178..7035)
/label="AmpR"
/note="β-lactamase"
promoter complement(7036..7140)
/label="AmpR promoter"
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