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pKD4-Hyg vector (V017648)

Basic Vector Information

Vector Name:
pKD4-Hyg
Antibiotic Resistance:
Ampicillin, Hygromycin
Length:
3601 bp
Type:
Gene-editing vector
Replication origin:
R6K γ ori
Host:
E. coli
Copy Number:
Low
5' Primer:
G36328-F1
Growth Strain(s):
S17-1λpir
Growth Temperature:
37℃

pKD4-Hyg vector Map

Copy Sequence View Map
pKD4-Hyg3601 bp60012001800240030003600AmpR promoterHygRFRT (minimal)R6K γ oriAmpRAmpR promoterrrnB T1 terminatorrrnB T2 terminator

pKD4-Hyg vector Sequence

LOCUS       .                       3601 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        458..562
                     /label="AmpR promoter"
     CDS             563..1585
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     protein_bind    complement(1779..1812)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(1861..2244)
                     /label="R6K γ ori"
                     /note="γ replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2317..3174)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3175..3279)
                     /label="AmpR promoter"
     terminator      3369..3455
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3547..3574
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"

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