pLenti6.2-ccdB-3×HA vector (V017647)

LOCUS       .                       8916 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        320..367
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             386..781
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             871..1104
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1176..1310
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1337..1472
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(1493..1511)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1521..1537)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1679..2134
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2160..2264
                     /label="AmpR promoter"
     CDS             2265..3122
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3296..3884
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4172..4193
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4208..4238
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4246..4262
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     4270..4286
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4307..4325
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4353..4579
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4580..4760
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4807..4932
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5425..5658
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5843..5887
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6036..6077
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     enhancer        6232..6534
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6535..6738
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        6783..6801
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6889..7013
                     /label="attR1"
                     /note="recombination site for the Gateway® LR reaction"
     promoter        7038..7068
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             7122..7778
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             8123..8425
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8469..8593)
                     /label="attR2"
                     /note="recombination site for the Gateway® LR reaction"
     CDS             8595..8621
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             8625..8651
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             8655..8681
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     promoter        8723..9222
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"