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pFB-OVP3H vector (V017646)

Basic Vector Information

Vector Name:
pFB-OVP3H
Antibiotic Resistance:
Ampicillin, Gentamycin
Length:
5464 bp
Type:
Protein expression
Replication origin:
ori
Host:
Insect cells
Promoter:
PH
5' Primer:
PH
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pFB-OVP3H vector Map

Copy Sequence View Map
pFB-OVP3H5464 bp60012001800240030003600420048005400f1 oriAmpR promoterAmpRoriTn7RGmRPc promoterpolyhedrin promoter6xHisSV40 poly(A) signalTn7L

pFB-OVP3H vector Sequence

LOCUS       .                       5464 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      2..457
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label="AmpR promoter"
     CDS             589..1446
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1620..2208
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     mobile_element  complement(2513..2737)
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2807..3337)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(3526..3554)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        3906..3997
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4721..4738
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    4954..5088
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(5117..5282)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

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