pdCas9-bacteria-ApmR vector (V017644)

LOCUS       .                       6818 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             95..895
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     terminator      925..1019
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      1133..1678
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1840..1867)
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     terminator      complement(1883..1954)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1981..6084)
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     promoter        complement(6120..6175)
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             6191..6814
                     /label="TetR"
                     /note="tetracycline repressor TetR"