Basic Vector Information
- Vector Name:
- pLP12-Gen
- Antibiotic Resistance:
- Chloramphenicol, Gentamycin
- Length:
- 3891 bp
- Type:
- Gene-editing vector
- Replication origin:
- R6K γ ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- BAD
- Growth Strain(s):
- S17-1λpir
- Growth Temperature:
- 30℃
pLP12-Gen vector Map
pLP12-Gen vector Sequence
LOCUS . 3891 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 27..311
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
promoter complement(1037..1055)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
rep_origin complement(1070..1458)
/label="R6K γ ori"
/note="γ replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
CDS complement(1618..2148)
/label="GmR"
/note="gentamycin acetyltransferase"
promoter complement(2337..2365)
/label="Pc promoter"
/note="class 1 integron promoter"
promoter 2492..2510
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
oriT 2664..2773
/label="oriT"
/note="incP origin of transfer"
CDS complement(3016..3891)
/label="araC"
/note="L-arabinose regulatory protein"
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