pSEVA231-Gen vector (V017636)

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Basic Vector Information

Vector Name:: pSEVA231-Gen

Antibiotic Resistance:: Gentamycin

Length:: 3211 bp

Type:: Gene-editing vector

Replication origin:: pBBR1 oriV

Host:: Bacteria

3' Primer:: rrnBT1-R

Growth Strain(s):: DH5a

Growth Temperature:: 30℃

pSEVA231-Gen vector Map

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pSEVA231-Gen vector Sequence

LOCUS       .                       3211 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    56..112
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        504..532
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             721..1251
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     oriT            1451..1559
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(1573..2232)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2233..3004)
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     terminator      complement(3119..3205)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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