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pKL2299 vector (V017635)

Basic Vector Information

Vector Name:
pKL2299
Antibiotic Resistance:
Gentamycin
Length:
29605 bp
Type:
Protein expression
Replication origin:
oriV
Host:
Bacteria
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pKL2299 vector Map

Copy Sequence View Map
pKL229929605 bp14002800420056007000840098001120012600140001540016800182001960021000224002380025200266002800029400virB2virB7virB9virB11virGFactor Xa sitetrfAoriVlambda t0 terminatorGmRPc promoter

pKL2299 vector Sequence

LOCUS       .                      29605 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3199..3561
                     /gene="virB2"
                     /label="virB2"
                     /note="Protein virB2 from Rhizobium radiobacter.
                     Accession#: P09776"
     CDS             7954..8118
                     /gene="virB7"
                     /label="virB7"
                     /note="Outer membrane lipoprotein virB7 from Rhizobium
                     radiobacter. Accession#: P0A3W4"
     CDS             8818..9696
                     /gene="virB9"
                     /label="virB9"
                     /note="Protein virB9 from Rhizobium radiobacter.
                     Accession#: P0A3W6"
     CDS             10869..11897
                     /gene="virB11"
                     /label="virB11"
                     /note="Protein VirB11 from Rhizobium radiobacter.
                     Accession#: P0A3F8"
     CDS             12032..12832
                     /gene="virG"
                     /label="virG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
     CDS             16059..16070
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             complement(26113..27258)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(27651..28182)
                     /label="oriV"
                     /note="incP origin of replication"
     terminator      complement(28294..28388)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(28662..29192)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(29381..29409)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"

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