pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327) vector (V017632)

LOCUS       .                       9403 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(join(9322..9403,1..998))
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(999..1076)
                     /label="lacI promoter"
     promoter        1385..1403
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1404..1428
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     RBS             1443..1465
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3078..4175
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     CDS             5652..5669
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      5736..5783
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      5820..6275
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(6370..7182)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7304..7892
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8078..8217)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(8322..8510)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(9285..9306)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."