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TUBB5-Halo vector (V017629)

Basic Vector Information

Vector Name:
TUBB5-Halo
Antibiotic Resistance:
Kanamycin
Length:
6207 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
5' Primer:
CMV-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

TUBB5-Halo vector Map

Copy Sequence View Map
TUBB5-Halo6207 bp30060090012001500180021002400270030003300360039004200450048005100540057006000CMV enhancerCMV promoterHaloTag®SV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

TUBB5-Halo vector Sequence

LOCUS       .                       6207 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        68..371
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1990..2877
                     /label="HaloTag®"
                     /note="modified bacterial dehalogenase that forms covalent
                     bonds with chloroalkane derivatives"
     polyA_signal    3002..3123
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3130..3585)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3612..3716
                     /label="AmpR promoter"
     promoter        3718..4075
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4110..4901
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    5136..5183
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5512..6100
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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