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pK18msB vector (V017628)

Basic Vector Information

Vector Name:
pK18msB
Antibiotic Resistance:
Kanamycin
Length:
3282 bp
Type:
Gene replacement vector
Replication origin:
ori
Host:
Bacteria
Selection Marker:
Neo/G418,SacB
3' Primer:
M13R
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pK18msB vector Map

Copy Sequence View Map
pK18msB3282 bp6001200180024003000NeoR/KanRSacBoriToriM13 revMCSM13 fwd

pK18msB vector Sequence

LOCUS       .                       3282 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             join(2910..3282,1..419)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             429..1847
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     oriT            complement(1867..1975)
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      2127..2715
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     2727..2743
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2752..2808
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2812..2828)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"

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