pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9-35S-DsRed2-NOS-Bar vector (V017625)

LOCUS       .                      15617 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(21..45)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_RNA        720..795
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        1431..2102
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             2372..6472
                     /label="Cas9"
                     /note="S. pyogenes Cas9, codon optimized for C. elegans
                     with synthetic introns"
     CDS             6940..7614
                     /label="DsRed2"
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     terminator      7624..8256
                     /label="E9 terminator"
                     /note="terminator and polyadenylation signal from the pea
                     rbcS-E9 gene"
     promoter        complement(8319..8349)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8364..8385)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8576..8755
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     CDS             8797..9345
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     terminator      9368..9620
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             9807..10598
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      10688..11276
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(11462..11602)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11946..12140)
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12209..13279)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13711..14337)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"