pSGKP-km-Hyg vector (V017624)

LOCUS       .                       6305 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        97..131
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        149..224
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     primer_bind     complement(257..273)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(332..348)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(356..372)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(380..410)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(425..446)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(734..1322)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1496..2308)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2309..2413)
                     /label="AmpR promoter"
     primer_bind     2887..2903
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2919..3941)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(4397..4499)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     primer_bind     4517..4533
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4598..6016)
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"