pCMV-3×HA-mCherry-Neo vector (V017618)

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Basic Vector Information

Vector Name:: pCMV-3×HA-mCherry-Neo

Antibiotic Resistance:: Kanamycin

Length:: 4795 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells

Selection Marker:: Neo/G418

Promoter:: CMV

5' Primer:: CMV-F

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pCMV-3×HA-mCherry-Neo vector Map

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pCMV-3×HA-mCherry-Neo vector Sequence

LOCUS       .                       4795 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             644..670
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             674..700
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             704..730
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             752..1459
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     polyA_signal    1583..1704
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1711..2166)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2193..2297
                     /label="AmpR promoter"
     promoter        2299..2656
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2691..3482
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    3717..3764
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4093..4681
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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