Basic Vector Information
- Vector Name:
- pMAK705-nifL-CmR-nifL-Kan
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8042 bp
- Type:
- Protein expression
- Replication origin:
- pSC101 ori
- Host:
- Bacteria
- Copy Number:
- Low
- 5' Primer:
- PUC57-F1
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
pMAK705-nifL-CmR-nifL-Kan vector Map
pMAK705-nifL-CmR-nifL-Kan vector Sequence
LOCUS . 8042 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
protein_bind 189..210
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 225..255
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 263..279
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
primer_bind 287..303
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 875..977
/label="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 978..1634
/label="CmR"
/note="chloramphenicol acetyltransferase"
primer_bind complement(2621..2637)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(3585..4397)
/label="KanR"
/note="aminoglycoside phosphotransferase"
promoter 4748..4852
/label="AmpR promoter"
CDS complement(6339..7286)
/label="Rep101(Ts)"
/note="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
rep_origin complement(7334..7556)
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
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