Basic Vector Information
- Vector Name:
- pET28-GFP
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5989 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- T7
- 5' Primer:
- T7
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pET28-GFP vector Map
pET28-GFP vector Sequence
LOCUS . 5989 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
terminator complement(26..73)
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/label="6xHis"
/note="6xHis affinity tag"
CDS complement(207..923)
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
protein_bind complement(967..991)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(992..1010)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 1319..1396
/label="lacI promoter"
CDS 1397..2476
/label="lacI"
/note="lac repressor"
protein_bind 2492..2513
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 3288..3476
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 3581..3720
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(3906..4494)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 4616..5428
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin complement(5523..5978)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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