Basic Vector Information
- Vector Name:
- pMAK705-Kan
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5794 bp
- Type:
- Protein expression
- Replication origin:
- pSC101 ori
- Host:
- E. coli
- Copy Number:
- Low
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
pMAK705-Kan vector Map
pMAK705-Kan vector Sequence
LOCUS . 5794 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
rep_origin 487..709
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
CDS 757..1704
/label="Rep101(Ts)"
/note="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
promoter complement(3191..3295)
/label="AmpR promoter"
CDS 3646..4458
/label="KanR"
/note="aminoglycoside phosphotransferase"
primer_bind 5406..5422
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 5423..5479
/label="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(5492..5508)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5516..5532)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(5540..5570)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(5585..5606)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
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