pBI121-35S-Chlo-mCherry vector (V017606)

LOCUS       .                      13747 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(148..516)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(549..658)
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     misc_feature    complement(2454..2478)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     rep_origin      complement(3124..3748)
                     /label="oriV"
                     /note="incP origin of replication"
     protein_bind    4823..4844
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5965..6672
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             6679..6696
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      6716..6968
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(7611..7635)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9234..10379)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10681..11472)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     mobile_element  11664..12431
                     /label="IS1"
                     /note="prokaryotic transposable element"
     rep_origin      complement(13132..13742)
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"