Basic Vector Information
- Vector Name:
- pT7-6×His-EGFP-ntt1(Bacteria)-6×His
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7585 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- T7
- 5' Primer:
- pEGFP-C-5'
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
- Expression Method:
- IPTG induction
pT7-6×His-EGFP-ntt1(Bacteria)-6×His vector Map
pT7-6×His-EGFP-ntt1(Bacteria)-6×His vector Sequence
LOCUS . 7585 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
rep_origin 12..467
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(563..1375)
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 1497..2085
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2271..2413)
/label="bom"
/note="basis of mobility region from pBR322"
CDS complement(2518..2706)
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(3481..3502)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3518..4597)
/label="lacI"
/note="lac repressor"
promoter complement(4598..4675)
/label="lacI promoter"
promoter 4984..5002
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5003..5027
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
RBS 5042..5064
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5083..5100
/label="6xHis"
/note="6xHis affinity tag"
CDS 5110..5127
/label="thrombin site"
/note="thrombin recognition and cleavage site"
CDS 5131..5847
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
CDS 7429..7446
/label="6xHis"
/note="6xHis affinity tag"
terminator 7513..7560
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
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