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pBoxCDGC-KMet-Nluc vector (V017593)

Basic Vector Information

Vector Name:
pBoxCDGC-KMet-Nluc
Antibiotic Resistance:
Kanamycin
Length:
4507 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
5' Primer:
CMV-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pBoxCDGC-KMet-Nluc vector Map

Copy Sequence View Map
pBoxCDGC-KMet-Nluc4507 bp600120018002400300036004200CMV enhancerCMV promoterNlucSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

pBoxCDGC-KMet-Nluc vector Sequence

LOCUS       .                       4507 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             658..1170
                     /label="Nluc"
                     /note="NanoLuc® luciferase"
     polyA_signal    1295..1416
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1423..1878)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1905..2009
                     /label="AmpR promoter"
     promoter        2011..2368
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2403..3194
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    3429..3476
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3805..4393
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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