pM-Cas12a vector (V017586)

LOCUS       .                      12991 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        96..766
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             853..876
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             907..927
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             952..4632
                     /label="LbCpf1"
                     /note="CRISPR-associated protein from Lachnospiraceae
                     bacterium ND2006 (Zetsche et al., 2015)"
     CDS             4633..4680
                     /label="nucleoplasmin NLS"
                     /note="bipartite nuclear localization signal from
                     nucleoplasmin"
     terminator      6060..6312
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6360..6384
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6512..7854)
                     /label="2μ ori"
                     /note="yeast 2μ plasmid origin of replication"
     promoter        8113..8394
                     /label="TRP1 promoter"
     CDS             8395..9066
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(9172..9627)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(9746..10891)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(11193..11984)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(12193..12824)
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    12904..12928
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"