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pS-cr:GFP vector (V017585)

Basic Vector Information

Vector Name:
pS-cr:GFP
Antibiotic Resistance:
Kanamycin
Length:
10153 bp
Type:
Gene-editing vector
Replication origin:
oriV
Host:
Plants
Copy Number:
Low
Promoter:
CaMV 35S
5' Primer:
pEGFP-C-5'
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pS-cr:GFP vector Map

Copy Sequence View Map
pS-cr:GFP10153 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000CaMV 35S promoter (enhanced)EGFPNOS terminatorRB T-DNA repeat2μ oriTRP1 promoterTRP1f1 oritrfAKanRoriVLB T-DNA repeat

pS-cr:GFP vector Sequence

LOCUS       .                      10153 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        96..766
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             complement(2244..2960)
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     terminator      3222..3474
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3522..3546
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3674..5016)
                     /label="2μ ori"
                     /note="yeast 2μ plasmid origin of replication"
     promoter        5275..5556
                     /label="TRP1 promoter"
     CDS             5557..6228
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(6334..6789)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(6908..8053)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(8355..9146)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(9355..9986)
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    10066..10090
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"

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