Basic Vector Information
- Vector Name:
- EGFP-Rab11a-7
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5343 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- pEGFP-C-5'
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
EGFP-Rab11a-7 vector Map
EGFP-Rab11a-7 vector Sequence
LOCUS . 5343 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 1..304
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 553..1269
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
polyA_signal 2071..2192
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2199..2654)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2681..2785
/label="AmpR promoter"
promoter 2787..3144
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3179..3970
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase from Tn5"
polyA_signal 4205..4252
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4581..5169
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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