pGPTVII-Bar-U-MatryoshCaMP6s vector (V017578)

LOCUS       .                      13710 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             343..1050
                     /label="LSSmOrange"
                     /note="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
     CDS             1495..1941
                     /label="calmodulin"
                     /note="calcium-binding protein"
     terminator      1961..2209
                     /label="HSP terminator"
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya
                     et al., 2010)"
     misc_feature    2374..2398
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2863..3510
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     oriT            4194..4303
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             4336..4704
                     /label="traJ"
                     /note="oriT-recognizing protein"
     rep_origin      4834..5545
                     /label="oriV"
                     /note="incP origin of replication"
     mobile_element  complement(6168..6935)
                     /label="IS1"
                     /note="prokaryotic transposable element"
     CDS             7127..7918
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             8220..9365
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     misc_feature    10964..10988
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     11606..11622
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(11635..11835)
                     /label="gene 7 terminator"
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     CDS             complement(11873..12421)
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(12472..12651)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"