Basic Vector Information
- Vector Name:
- pCMV-mEGFP-2×linker-MCS-HA-Neo
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4769 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- PEGFP-C-5
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCMV-mEGFP-2×linker-MCS-HA-Neo vector Map
pCMV-mEGFP-2×linker-MCS-HA-Neo vector Sequence
LOCUS . 4769 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 61..364
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/label="mEGFP"
/note="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
CDS 1399..1425
/label="HA"
/note="HA (human influenza hemagglutinin) epitope tag"
polyA_signal 1557..1678
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1685..2140)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2167..2271
/label="AmpR promoter"
promoter 2273..2630
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2665..3456
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase from Tn5"
polyA_signal 3691..3738
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4067..4655
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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