Cart 2

Sfp pet29b C-terminal His Tag vector (V017572)

Basic Vector Information

Vector Name:
Sfp pet29b C-terminal His Tag
Antibiotic Resistance:
Kanamycin
Length:
5910 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Promoter:
T7
5' Primer:
T7
Growth Strain(s):
DH5a
Growth Temperature:
37℃
Expression Method:
IPTG induction

Sfp pet29b C-terminal His Tag vector Map

Copy Sequence View Map
Sfp pet29b C-terminal His Tag5910 bp60012001800240030003600420048005400f1 oriKanRoribomropCAP binding sitelacIlacI promoterT7 promoterlac operatorRBSsfp6xHisT7 terminator

Sfp pet29b C-terminal His Tag vector Sequence

LOCUS       .                       5910 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1497..2085
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2271..2413)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3481..3502)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4598..4675)
                     /label="lacI promoter"
     promoter        4988..5006
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5007..5031
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     RBS             5046..5068
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5076..5747
                     /gene="sfp"
                     /label="sfp"
                     /note="4'-phosphopantetheinyl transferase Sfp from Bacillus
                     subtilis (strain 168). Accession#: P39135"
     CDS             5754..5771
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      5838..5885
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"

This page is informational only.