Cart 2

pET30a-PmerR-Curli vector (V017567)

Basic Vector Information

Vector Name:
pET30a-PmerR-Curli
Antibiotic Resistance:
Kanamycin
Length:
8700 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Copy Number:
Low
3' Primer:
LacI-R
Growth Strain(s):
DH5a
Growth Temperature:
37℃
Expression Method:
IPTG induction

pET30a-PmerR-Curli vector Map

Copy Sequence View Map
pET30a-PmerR-Curli8700 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400f1 oriKanRoribomropCAP binding sitelacIlacI promoterT7 terminator

pET30a-PmerR-Curli vector Sequence

LOCUS       .                       8700 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1497..2085
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2271..2413)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3481..3502)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4598..4675)
                     /label="lacI promoter"
     terminator      8628..8675
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"

This page is informational only.