Basic Vector Information
- Vector Name:
- pDL278-Kan
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6781 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Gram-positive bacteria, Enterococcus faecalis
- Copy Number:
- Low
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pDL278-Kan vector Map
pDL278-Kan vector Sequence
LOCUS . 6781 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
rep_origin complement(237..825)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 1682..2494
/label="KanR"
/note="aminoglycoside phosphotransferase"
terminator 5362..5405
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator complement(5701..5728)
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(5820..5906)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 6380..6396
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 6397..6453
/label="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(6466..6482)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6490..6506)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(6514..6544)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(6559..6580)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
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