pBI121-35S-MCS1-P2A-MCS2-T2A-MCS3-IRES-MCS4-mCherry-Neo vector (V017565)

LOCUS       .                      14323 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(148..516)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(549..658)
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     misc_feature    complement(2454..2478)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(3700..4324)
                     /label="oriV"
                     /note="incP origin of replication"
     protein_bind    4823..4844
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5828..5884
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             5897..5950
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     misc_feature    5966..6539
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             6565..7272
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    complement(8187..8211)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9810..10955)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(11257..12048)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     mobile_element  12240..13007
                     /label="IS1"
                     /note="prokaryotic transposable element"
     rep_origin      complement(13708..14318)
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"