pGD-γb vector (V017563)

Basic Vector Information

Vector Name:
pGD-γb
Antibiotic Resistance:
Kanamycin
Length:
7707 bp
Type:
Protein expression
Replication origin:
ori
Host:
Plants
Promoter:
35S
5' Primer:
35S
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pGD-γb vector Map

pGD-γb7707 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500LB T-DNA repeatCaMV 35S promoterNOS terminatorRB T-DNA repeatpVS1 StaApVS1 RepApVS1 oriVbomoriKanR

pGD-γb vector Sequence

LOCUS       .                       7707 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    5..29
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        179..524
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1159..1410
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1432..1456
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2757..3383
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             3815..4885
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4954..5148
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5492..5632
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5818..6406)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6496..7287)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"

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