Basic Vector Information
- Vector Name:
- pGD-γb
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7707 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Plants
- Promoter:
- 35S
- 5' Primer:
- 35S
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pGD-γb vector Map
pGD-γb vector Sequence
LOCUS . 7707 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_feature 5..29
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
promoter 179..524
/label="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
terminator 1159..1410
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 1432..1456
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 2757..3383
/label="pVS1 StaA"
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 3815..4885
/label="pVS1 RepA"
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 4954..5148
/label="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 5492..5632
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(5818..6406)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6496..7287)
/label="KanR"
/note="aminoglycoside phosphotransferase"
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