6xHis-MBP-BrCas12b-FNLDTA vector (V017559)

LOCUS       .                       9670 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        209..227
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    228..252
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     RBS             267..289
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             308..325
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             335..1435
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     CDS             1448..1468
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             4742..4759
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      4826..4873
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      4910..5365
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(5461..6273)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6395..6983
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(7169..7308)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(7413..7601)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(8376..8397)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(8413..9492)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(9493..9570)
                     /label="lacI promoter"