Basic Vector Information
- Vector Name:
- pJ241-FLEX
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3063 bp
- Replication origin:
- ori
- Host:
- Broad host
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pJ241-FLEX vector Map
pJ241-FLEX vector Sequence
LOCUS . 3063 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
primer_bind 89..105
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 575..608
/label="FRT (minimal)"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
protein_bind 655..688
/label="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
protein_bind 759..792
/label="lox2272"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
compatible with each other, but incompatible with loxP or
loxN sites (Lee and Saito, 1988)."
protein_bind complement(799..832)
/label="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
protein_bind complement(895..928)
/label="lox2272"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
compatible with each other, but incompatible with loxP or
loxN sites (Lee and Saito, 1988)."
protein_bind complement(945..978)
/label="FRT (minimal)"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
CDS 1363..2169
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 2294..2882
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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