Basic Vector Information
- Vector Name:
- pBAD Myc-HisB-Kan
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4047 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- BAD
- 5' Primer:
- PBAD30.F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
- Expression Method:
- Arabinose induction
pBAD Myc-HisB-Kan vector Map
pBAD Myc-HisB-Kan vector Sequence
LOCUS . 4047 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 1..285
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 375..404
/label="Myc"
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 420..437
/label="6xHis"
/note="6xHis affinity tag"
terminator 663..749
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 841..868
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 887..978
/label="AmpR promoter"
CDS 979..1791
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 1965..2553
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2739..2879)
/label="bom"
/note="basis of mobility region from pBR322"
CDS complement(3146..4021)
/label="araC"
/note="L-arabinose regulatory protein"
This page is informational only.