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pJL1-sfGFP vector (V017553)

Basic Vector Information

Vector Name:
pJL1-sfGFP
Antibiotic Resistance:
Kanamycin
Length:
2486 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Promoter:
T7
5' Primer:
T7
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pJL1-sfGFP vector Map

Copy Sequence View Map
pJL1-sfGFP2486 bp600120018002400RBST7 promoteroriKanRT7 terminatorStrep-Tag IIGFP(1-10)

pJL1-sfGFP vector Sequence

LOCUS       .                       2486 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(join(1950..2486,1..105))
                     /label="GFP(1-10)"
                     /note="fragment of superfolder GFP consisting of the first
                     10 β-strands"
     RBS             complement(113..135)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(167..185)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(213..801)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             923..1735
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      complement(1756..1803)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(1872..1895)
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin®, an engineered form
                     of streptavidin"

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