Cart 2

pTD103luxI vector (V017552)

Basic Vector Information

Vector Name:
pTD103luxI
Antibiotic Resistance:
Kanamycin
Length:
4849 bp
Type:
Signal Pathway Reporter Vectors
Replication origin:
ori
Host:
E. coli
Copy Number:
Low
Promoter:
Luxlp
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pTD103luxI vector Map

Copy Sequence View Map
pTD103luxI4849 bp6001200180024003000360042004800luxRssrA tagrrnB T1 terminatorluxRluxIssrA tagrrnB T1 terminatororilambda t0 terminatorNeoR/KanR

pTD103luxI vector Sequence

LOCUS       .                       4849 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(10..759)
                     /gene="luxR"
                     /label="luxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             986..1018
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      1060..1146
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1184..1933)
                     /gene="luxR"
                     /label="luxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             2160..2738
                     /gene="luxI"
                     /label="luxI"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             2739..2771
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      complement(2795..2838)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3043..3631)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(3719..3813)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3847..4638)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"

This page is informational only.