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SpyTag003-mKate2 vector (V017550)

Basic Vector Information

Vector Name:
SpyTag003-mKate2
Antibiotic Resistance:
Kanamycin
Length:
6024 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Copy Number:
Low
Promoter:
T7
5' Primer:
T7
Growth Strain(s):
DH5a
Growth Temperature:
37℃
Expression Method:
IPTG induction

SpyTag003-mKate2 vector Map

Copy Sequence View Map
SpyTag003-mKate26024 bp30060090012001500180021002400270030003300360039004200450048005100540057006000RBSmKate26xHisT7 terminatorf1 oriKanRoribomropCAP binding sitelacIlacI promoterT7 promoterlac operator

SpyTag003-mKate2 vector Sequence

LOCUS       .                       6024 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     RBS             15..37
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             117..809
                     /label="mKate2"
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     CDS             828..845
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      929..976
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1013..1468
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1563..2375)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2497..3085
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3271..3410)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3515..3703)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(4478..4499)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4515..5594)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5595..5672)
                     /label="lacI promoter"
     promoter        5981..5999
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6000..6024
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."

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