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pTP1005 vector (V017549)

Basic Vector Information

Vector Name:
pTP1005
Antibiotic Resistance:
Kanamycin
Length:
4675 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Promoter:
T5
3' Primer:
pQE-R
Growth Strain(s):
DH5a
Growth Temperature:
37℃
Expression Method:
IPTG induction

pTP1005 vector Map

Copy Sequence View Map
pTP10054675 bp600120018002400300036004200T5 promoterFactor Xa sitelambda t0 terminatorKanRrrnB T1 terminatorCAP binding sitelacIlacIq promoterbomori

pTP1005 vector Sequence

LOCUS       .                       4675 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..45
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     CDS             complement(772..783)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     terminator      911..1005
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             1064..1876
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      2098..2184
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(2243..2264)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2280..3359)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(3360..3437)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    3629..3769
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3955..4543)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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