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pmTurquoise2-Golgi vector (V017548)

Basic Vector Information

Vector Name:
pmTurquoise2-Golgi
Antibiotic Resistance:
Kanamycin
Length:
4904 bp
Type:
Subcellular localization
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
3' Primer:
Sv40-polyA-R
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pmTurquoise2-Golgi vector Map

Copy Sequence View Map
pmTurquoise2-Golgi4904 bp6001200180024003000360042004800CMV enhancerCMV promotermTurquoise2SV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

pmTurquoise2-Golgi vector Sequence

LOCUS       .                       4904 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        9..312
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        313..516
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             798..1514
                     /label="mTurquoise2"
                     /note="enhanced monomeric variant of CFP (Goedhart et al.,
                     2012)"
     polyA_signal    1640..1761
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1768..2223)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2250..2354
                     /label="AmpR promoter"
     promoter        2356..2713
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2748..3539
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    3774..3821
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4150..4738
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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