Basic Vector Information
- Vector Name:
- pEGFP-C1+SKL
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4722 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- pEGFP-C-5'
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pEGFP-C1+SKL vector Map
pEGFP-C1+SKL vector Sequence
LOCUS . 4722 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 61..364
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
polyA_signal 1510..1631
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1638..2093)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2120..2224
/label="AmpR promoter"
promoter 2226..2583
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2618..3409
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase from Tn5"
polyA_signal 3644..3691
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4020..4608
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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