pRSFDuet-1_VNp-mNeongreen-Erythropoietin vector (V017546)

LOCUS       .                       5164 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    3..27
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             83..100
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        214..232
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             432..1139
                     /label="mNeonGreen"
                     /note="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
     CDS             1668..1685
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1701..1745
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      1797..1844
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(2077..2889)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2890..2981)
                     /label="AmpR promoter"
     rep_origin      complement(2997..3746)
                     /label="RSF ori"
                     /note="Plasmids containing the RSF 1030 origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(3908..3929)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3945..5024)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5025..5102)
                     /label="lacI promoter"