pCMV-EGFP-MCS-Myc-FLAG-Neo vector (V017542)

LOCUS       .                       5627 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     enhancer        343..722
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1010..1726
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             1841..1864
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     primer_bind     complement(1884..1900)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     polyA_signal    1911..2533
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     rep_origin      complement(2682..3270)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(3599..3646)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3881..4672)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(4707..5064)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(5066..5170)
                     /label="AmpR promoter"
     rep_origin      5197..5652
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"